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What Is Needed To Express A Gene When Placed On A Dna Strand?

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1. What Is Needed To Express A Gene When Placed On A Dna Strand?

A Promoter Region Is Needed For The Gene To Be Expressed To Produce Proteins.
A Dna Strand Is Placed In A Soln' Of Primers And Dntp And A Small Amount Of Ddntp Which Are Lacking An Additional Oh Group On Its 3' Carbon. This Makes Dna Polymerase Stop When It Encounters A Ddnucleotide, Because There Is No Place For The Next Phosphate To Bind. Then The Dna Is Ran In A Gel And You Will Have Groups Of Dna Strands That Terminate On Each Nucleotide. The Gel Is Read From The Bottom Up For The Sequence That Was Replicated Via Pcr. Then You Have To Have The Reverse Of The Sequence Because The Original Strand Does Not Have The Ddntp's Incorperated Into Them, The Complimentary Strand Does. You Have To Write The Reverse Of What Is Found On The Gel To Have The Original Sequence.
Mental Retardation, Characteristic Facial Features, Large Testes
Pt, Aptt

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What Is Needed To Express A Gene When Placed On A Dna Strand?

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