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Bioinformatics Practice Test Questions: Collecting & Storing Sequences in Laboratory
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Here are some steps for collecting and storing sequences in a laboratory: Nucleic acid extraction and isolation Library preparation Clonal amplification and sequencing Data analysis using bioinformatics Topics include: DNA & Genomic Sequencing, Sequencing cDNA Libraries of Expressed Genes & Submission of Sequences to the Databases, Sequence Formats & Computer Storage of Sequences, Multiple Sequence Formats, Storage of Information in a Sequence Database, Using the Database Access Program ENTREZ, Genome Anatomy, Sequence Assembly and Gene Identification & Comparative... Show more
Bioinformatics Practice Test Questions: Collecting & Storing Sequences in Laboratory
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25 Questions

1. For a protein search, which of the following is the wrong choice for fields?
2. Computational resources can facilitate the analysis of bacterial genomes.
3. Introns can remain at a corresponding position in a eukaryotic gene for long periods of evolutionary time.
4. Sequencing of genomes depends on the assembly of a large number of DNA reads into a linear, contiguous DNA sequence.
5. Which of the given statements is incorrect?
6. In Metabolic pathways and regulation, as genes are identified in a new genome sequence, some will be found that are known to act sequentially in a metabolic pathway or to have a known role in gene regulation in other organisms.
7. In case of genome sequence assembly which of the given statement is incorrect?
8. Which of the given statements is incorrect about Grouping Sequences?
9. Which of the following is wrong about FASTA Sequence Format?
10. The program returns the number of matches found and provides an opportunity to narrow this list by including more terms.
11. Eukaryotic genes that encode proteins are interrupted by ________
12. Which of the following is incorrect about ENTREZ?
13. In Proteolysis and fragment sequencing, Protein spots may be excised from a two-dimensional protein gel and subjected to a combination of amino acid sequencing and cleavage analyses using the techniques of mass spectrometry and high-pressure liquid chromatography.
14. Screening the predicted protein sequences against ______ library confirms the prediction and expression of the gene.
15. Which of the given statement is incorrect about Functional Genomics?
16. Which of the following is untrue about Shotgun Sequencing?
17. To assist in finding suitable terms, for each field, ENTREZ provides a list of index entries.
18. (Transposable Elements) TEs have the potential to _____ in number until they comprise a ____ proportion of the genome sequence.
19. The sequences of satellite DNA fall into different types, each with a different repeat unit of length _____
20. For computer analysis of proteins, it is more convenient to use single-letter than three letter amino acid codes.
21. Which of the following is wrong about Genetics Computer Group Sequence Format?
22. The all-against-all analyses provide an indication as to the number of protein/gene families in an organism. This number represents the core proteome of the organism from which all biological functions have diversified.
23. An alternative method is to sequence all the subclones, produce a computer database of the sequences, and then have the computer assemble the sequences from the overlaps that are found.
24. Which of the given statements is incorrect about ASCII and Hexadecimal?
25. The translated sequence can then be compared to a database of protein sequences with the hope of finding a strong similarity to a protein of known function, and hence to identify the function of the cloned EST.

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