The conventional determination of open reading methods identify only typical genes and tend to miss atypical genes in which the rule of codon bias is not strictly followed.

Gene and promoter prediction is a crucial research area in bioinformatics. Accurate prediction of promoters is important for understanding gene function and regulation, and for interpreting gene expression patterns.

Topics include: Categories of Gene Prediction Programs, Gene Prediction in Prokaryotes, & Gene Prediction in Eukaryotes.