Membership in a stable complex also differs from transient interaction in terms of evolutionary constraints upon sequence divergence.

The yeast two-hybrid method allows the mapping of binary or pair-wise interactions, protein chips are suited to detect protein–protein, pro- tein–lipid and other protein–ligand interactions.

Topics include: Protein – Protein Interactions, Structural Analyses of Domain Interactions, & The Use of Gene Order & Phylogeny to Predict Protein – Protein Interactions.