Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

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Main subjects taught in graduate / degree level biotechnology course are: Introductory Biological Chemistry, Animal and Plant Physiology, Recombinant DNA Technology, DNA Typing, Proteomics and Beyond, Biodiversity and Taxonomy, Microbiology and Macromolecule, and Biostatistics.

Job opportunities: With a degree in Biotechnology, you can become a scientist, researcher, or entrepreneur working in fields such as medicine, agriculture, and food production. With a BSc in Biotechnology, you can work in various industries, including agriculture, medicine, and food production.


Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to