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MCQs for Biotechnology: Principles and Processes
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MCQs for Biotechnology: Principles and Processes
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25 Questions

1. pBR322 was the first artificial cloning vector to be constructed. What does 'BR' stands for ?
2. Which of the following bacteria is used as a vector for plant genetic engineering ?
3. Which of the following statements is not correct regarding EcoRI restriction endonuclease enzyme ?
4. The correct sequence of different steps of polymerase chain reaction is
5. In agarose gel electrophoresis, DNA molecules ar. separated on the basis of their
6. Which of the following is not a cloning vector ?
7. The first restriction endonuclease isolated was
8. Micro-injection is a method used to
9. a crown gall bacterium, is called as 'natural genetic engineer' of plants.
10. If a plasmid vector is digested with EcoRI at a single site, then
11. While isolating DNA from bacteria, which of the following enzymes is not used ?
12. Which of the following is not used to transfer the recombinant DNA into the host ?
13. Which of the following statements does not hold true for restriction enzyme ?
14. An antibiotic resistance gene in a vector usually helps in the selection of
15. Enzyme 'Taq polymerase' used in PCR, has been isolated from bacterium
16. Which of the following steps are catalysed by Taq polymerase in a PCR reaction ?
17. Identify the palindromic sequence in the following.
18. The transfer of genetic material from one bacterium to another through the mediation of a vector like virus is termed as
19. Which of the following is not required in the preparation of a recombinant DNA molecule ?
20. An enzyme catalysing the removal of nucleotides from the ends of DNA is
21. Which of the following is not a source of restriction endonuclease ?
22. The term 'molecular scissors' refers to
23. ce gene (tetR) has recognition site for which of the following re. In pBR322, tetracycline resistantriction endonuclease ?
24. A device in which large volume of living cells ar. cultured in order to get a specific product is called
25. The term ’chemical knife’ refers to

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