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Genetic Engineering Practice Test: Basics of Genetic Engineering
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Basics of Genetic Engineering topics include: Restriction endonucleases and phosphatases, polymerases, exonucleases, categories and enzymes for ligation, purification of plasmid dna, transformation, gel electrophoresis oligonucleotide and microarrays. Genetic engineering, also known as genetic modification, is a process that alters an organism's DNA. It involves using laboratory tools to insert, alter, or cut out pieces of DNA that contain one or more genes of interest.  Here are some basic concepts of genetic engineering: Recombinant DNA: A technology that involves isolating a gene from a... Show more
Genetic Engineering Practice Test: Basics of Genetic Engineering
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25 Questions

1. The specificity of an enzyme is affected by the concentration of buffer used. This phenomenon is termed as:
2. If the amount of agarose added is more, the molecular under analysis should have the following characteristic:
3. Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?
4. Which of the following components settles at the bottom?
5. Chromosomal or genomic DNA is separated by ____________
6. Often the DNA or RNA needs to be removed from the gel to the solid support. The process can be termed as ____________
7. What is the mode of action of exonuclease III?
8. The charge present on the DNA backbone is negative. The force required to accelerate the molecules towards anode is directly proportional to the number of ____________
9. The sticky ends are held together by which type of bonds?
10. Why is DNase preferred over restriction endonuclease in some cases?
11. What is the kinetics of the intramolecular and intermolecular ligation reactions?
12. A sequence is having two ends, 5’ and 3’. Which of the following statements is correct regarding the nature of the ends?
13. If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried out?
14. If all the nucleotides are present with equal frequencies and at random, what are the chances of having a particular four nucleotide long motif?
15. The recognition sequence of Sau3A is 5’ |GATC 3’ and that for DpnI is 5’ GA|TC 3’. Which of the statements is true?
16. Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it?
17. Introduction of DNA molecules into the recipient organism is termed as ____________
18. Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is?
19. Phage based recombination systems are used for ____________
20. Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is ____________
21. Electroporation is also used for taking up the DNA by the cells. It constitutes of ____________
22. The recognition sequence is at times palindromic in nature. Which of the following statements is correct with respect to it?
23. Force is defined as mass per unit acceleration. As the number of phosphate molecules increases, the charge also increases which increases the force required. The acceleration is dependent on the size of the molecules.
24. Which of the following statement is correct regarding partial digestion?
25. Many bacterial species are having a natural ability to take up the exogenous DNA material. Which of the statement is not correct in regard to it?