Genetic Engineering Practice Test: Other Vector Systems for E. Coli — Flashcards

Other Vector Systems for E. Coli topics include: BAC (bacterial artificial chromosomes) vector, bacteriophage lambda, cloning in lambda, lambda zap and cosmids.

Here are some other vector systems for E. coli:
Plasmids: These can serve as vectors, carrying a gene to an organism. The gene can then be replicated, transcribed, and translated within the host organism.
Cloning vectors: These include plasmids, bacteriophages, cosmids, and bacterial artificial chromosomes (BACs).
Shuttle vectors: These are used for protein production in other organisms such as E. coli. 

E. coli contains many standard cloning vectors, including:
PBR322: Contains origin of replication (ori)
PACYC177: Contains ampicillin resistance gene
PBR324: Contains ampicillin resistance gene
PRK 64.6: Contains ampicillin resistance gene 

Cloning vectors usually contain systems to permit maintenance and function in E. coli. Sometimes, they have other characteristics that allow them to persist in organisms other than E. coli.