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The structure of adenosine triphosphate (ATP) is shown below (Figure 1). ATP is hydrolyzed to ADP and inorganic phosphate when in equilibrium with water, and the high energy of this molecule comes from two high-energy phosphoanhydride bonds. Each bond has a ΔG of −30.5 kJ/mol. Therefore, the free energy released by hydrolysis of ATP to ADP and inorganic phosphate under standard conditions, ∆G°´, is equal to −30.5 kJ/mol at pH 7.0.
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