The translated sequence can then be compared to a database of protein sequences with the hope of finding a strong similarity to a protein of known function, and hence to identify the function of the cloned EST.

Here are some steps for collecting and storing sequences in a laboratory: Nucleic acid extraction and isolation Library preparation Clonal amplification and sequencing Data analysis using bioinformatics Topics include: DNA & Genomic Sequencing, Sequencing cDNA Libraries of Expressed Genes & Submission of Sequences to the Databases, Sequence Formats & Computer Storage of Sequences, Multiple Sequence Formats, Storage of Information in a Sequence Database, Using the Database Access Program ENTREZ, Genome Anatomy, Sequence Assembly and Gene Identification & Comparative... Show more

Here are some steps for collecting and storing sequences in a laboratory:
Nucleic acid extraction and isolation
Library preparation
Clonal amplification and sequencing
Data analysis using bioinformatics

Topics include: DNA & Genomic Sequencing, Sequencing cDNA Libraries of Expressed Genes & Submission of Sequences to the Databases, Sequence Formats & Computer Storage of Sequences, Multiple Sequence Formats, Storage of Information in a Sequence Database, Using the Database Access Program ENTREZ, Genome Anatomy, Sequence Assembly and Gene Identification & Comparative Genomics.

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