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BIO311 Final Exam (Molecular Biology)
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MCQs on the structure and function of DNA and RNA and how these nucleic acids are used to form proteins, and their importance in genetics and inheritance. Also covers DNA replication, transcription, gene expression, mutation and repair, recombination, molecular techniques, and appropriate molecular models.
 

BIO311 Final Exam (Molecular Biology)
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25 Questions

1. Electrophoresis is separating molecules based on their______.
2. Repressible operons are typical in _____, e.g., the _____. And inducible operons are typical in _____, e.g., the _____.
3. Use the standard genetic code table to determine which mRNA codes for the following protein: Met-Arg-Gly-Tyr-Ala-Arg.
4. ______ is perhaps the most common eukaryote in molecular biology labs. It is also widely used in commercial fermentation applications.
5. The mismatch repair system has to recognize the ______ strand and repair mutations only in this strand.To achieve this, bacteria is utilizing the postreplicational delay in ______.
6. _____ proteins keep the template DNA and the DNA polymerases associated, which is essential for an uninterrupted DNA strand synthesis.
7. When tryptophan is present in sufficient quantities, the 'font-style:italic;'>trp operon is _____, since the _____ binds to the operator within the operon.
8. Riboswitches are _____ that can silence protein expression if a _____ binds to them.
9. The key of the dideoxy DNA sequencing method is the missing ______.
10. DNA polymerases are 'processive' enzymes.Processive enzymes catalyze subsequent chemical reactions on a polymer substrate _____, while dispersive enzymes _____ between two subsequent catalytic reactions.
11. DNA is typically _____, while RNA is _____.
12. Only part of the mRNA molecule is translated into protein.This translated region is called
13. Restriction enzymes are ______ that ______ the DNA at specific ______.
14. In the presence of a selenocysteine insertion (SECIS) element in the mRNA, the UCA anticodon translates into selenosysteine in many redox enzymes.In the absence of the SECIS element, the UCA is the umber stop codon.Which mRNA codon would the UCA anticodon pair with?Keep in mind that base-pairing occurs between antiparallel nucleic acids.
15. ______ is perhaps the most common prokaryote in molecular biology labs. It is also widely used by the biotech industry to manufacture proteins, e.g., insulin.
16. ______ is a favorite model organism from the early days of genetics. It taught us how to link gene distance and genetic recombination frequencies, body plan development, and molecular mechanisms of learning and memory, just to mention a few.
17. During DNA replication, the _____ is synthesized continuously, while the _____ is synthesized in pieces and linked by a ligase later on.
18. The anticodon for tryptophan (Trp) is 5'-CCA-3'.Which mRNA codon would the Trp-tRNA pair with?
19. Which of the following is true regarding open reading frames?
20. Which of the following statement is true?
21. In 'font-style:italic;'>E. coli, the gene 'font-style:italic;'>lacI codes the 'font-style:italic;'>lac operon repressor. Which of the following statements is true about an 'font-style:italic;'>E. coli strain, which is 'font-style:italic;'>lacI-, that is null mutant for 'font-style:italic;'>lacI.
22. A cloning plasmid typically has a(n) ______, a ______, and a______.
23. DNA replication is _____.
24. Histone deacetylation enhances chromosome _____, thus the chromosomal segment cannot be _____.
25. Which of the following is not a DNA damage type?

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