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Genetic Engineering Practice Test: Use of Cloned DNA
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Use of Cloned DNA topics include: Promoters, synthesis of rna and proteins, expression in vivo, fusion proteins, gene function and its traping. DNA cloning is used to create multiple copies of a gene or other piece of DNA. It can be used for a variety of purposes, including: Studying genes and their protein products: This can be important in human disease research. Creating genetically modified organisms: These can be used to study human disease, such as knock-out mice. Producing vaccines: For example, the hepatitis B vaccine. Producing transgenic plants: With improved qualities like... Show more
Genetic Engineering Practice Test: Use of Cloned DNA
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25 Questions

1. His tagged proteins can be eluted using EDTA or a pH gradient from the matrix.
2. A considerable amount of modification can take place in the ______ region of RNA in _______ before translation. It affects the yield of proteins.
3. It is also possible to generate a fusion protein in which ______ is encoded by the vector.
4. Small quantities of radiolabelled RNA can be produced by translation in vitro which is done by transcription in vitro.
5. In fusion proteins, the reading frame in a vector is out of phase with the insert.
6. Lon protease is used for degradation of foreign proteins in E.coli cells. It is active against which type of proteins?
7. What is the use of adding micrococcal nuclease in the reticulocyte cells?
8. The hybrid intron is spliced from the transcript using the splice acceptor site in the vector.
9. As the activity of T7 polymerase is reduced by lysosome, what is an effect on the rate of synthesis after induction?
10. For the production of unlabelled and huge amount of proteins, which of the following is true?
11. Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector.
12. Isolation of RNA can be carried out easily from bacterial cells.
13. What is the function of T7 lysozyme?
14. By selecting the appropriate polymerase to activate the promoter ________ can be carried out _______
15. Lon mutation can lead to ______ and _______
16. Guanylyl transferase and GTP are used for?
17. Choose the correct statement for rifampcin.
18. If detection of enhancers is done it is called as ______ and for detection of transcribed sequences, it is called as ______
19. ______ fluorescent protein should be used for determination of _______ of subcellular components.
20. Cyanogen bromide is used for cleavage of junctions. It cleaves after ________ residues.
21. Lambda PL promoter is used in which vectors?
22. Insertion of vector into intron gives rise to ________ it contains a part coming from intron and a part from ______
23. When lacUV5 control system is used, addition of IPTG _______ the expression of _________
24. Expression of T7 promoter- lac operator hybrid requires ___________
25. If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.