Which of the following lists the steps for cDNA library preparation in the correct order? - - i. Partially degrade the mRNA with RNase H - ii. Isolate mRNA from a specific cell/tissue with an oligo-dT primer that hybridizes to a poly-A tail - iii. Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers - iv. Ligate cDNAs into a cloning vector - v. Create single-stranded DNA molecule complementary to the mRNA using - reverse transcriptase

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1. Which of the following lists the steps for cDNA library preparation in the correct order? - - i. Partially degrade the mRNA with RNase H - ii. Isolate mRNA from a specific cell/tissue with an oligo-dT primer that hybridizes to a poly-A tail - iii. Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers - iv. Ligate cDNAs into a cloning vector - v. Create single-stranded DNA molecule complementary to the mRNA using - reverse transcriptase