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MCAT Biology Practice Test 4: Molecular Biology
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MCAT Biology Practice Test 4: Molecular Biology
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25 Questions

1. In order to isolate genes for cloning into another organism, DNA is frequently fragmented by enzymes. In order to purify these fragments, what is done next?
2. Bacterial transcription requires the presence of what is known as a sigma (σ-) subunit. Why is this component important?
3. DNA-dependent RNA polymerase functions
4. The cumulative length of DNA within a single human nucleus, if laid end to end, would be about 1.8 meters. Of that, approximately how much codes for human proteins?
5. During PCR, what mechanism is used to separate the complementary DNA strands from each other?
6. A frame-shift mutation within a region coding for an intron
7. Which of the following is the best distinction between DNA and RNA?
8. The phases of translation consist of
9. DNA ligase is used to
10. Intact ribosomes are assembled
11. In order to help determine which material in the previous question was responsible for the pigment change, which technique could eliminate one of the possibilities?
12. The function of which of the following is utilized in lagging-strand synthesis but not leading-strand synthesis?
13. Which of the following increases fidelity during replication?
14. Ultraviolet light is carcinogenic because
15. Which of the following codon pairs would most likely code for the same amino acid because of the wobble in the genetic code?
16. During translation, how is the subsequent amino acid transferred from the tRNA that brought it into the ribosome to the nascent protein strand?
17. Which of the following mutations is most likely to produce a lethal condition?
18. What enzyme(s) is/are used by researchers to excise genes of interest?
19. Which of the following sequences would hybridize the strongest to the sequence 5′-ATTTGGGCCAATGGGCCCTTTAA-3′?
20. If the base sequence triplet AAC was found on the DNA sense strand, what would be the resulting amino acid added within the ribosome?

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21. Which of the following mechanisms of DNA repair are mediated by the recA protein?
22. RNA is believed to be a more primitive molecule than DNA because
23. If a nucleic acid was found in a cell with a long terminal repetitive sequence of adenines, then it would probably be
24. Ribosomes are responsible for what cellular activity?
25. Which base pairing below represents the strongest binding?

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