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Microbiology Practice Test: Pure Cultures and Cultural Characteristics
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Pure Cultures and Cultural Characteristics topics include: Selective methods, pure cultures and cultural characteristics. A pure culture is a laboratory culture that contains only one species of organisms. A pure culture is free of all other microorganisms, including other cells of the same organism. However, most pure cultures will have the same general biochemical characteristics as the original organism.  A pure culture may originate from a single cell or single organism, in which case the cells are genetic clones of one another. A pure culture can be derived from a mixed culture through... Show more
Microbiology Practice Test: Pure Cultures and Cultural Characteristics
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25 Questions

1. Surface texture of strains are related to virulence.
2. The American Type Culture Collection, located in Rockville, Maryland in the year 1980 has a collection of bacterial strain in what numbers?
3. Growth in broth cultures occurs mainly in the form of__________
4. In physical method of selection, endospore-forming bacteria can be obtained by heating the mixed culture to _____________
5. Dilution of the mixed culture is a common step in all the three methods: the streak-plate, the spread-plate and the pour-plate technique.
6. Which of the following is a function of cryoprotective agents?
7. Which of the following are not performed in lyophilization?
8. Isolation of pure culture refers to ___________
9. Pyoverdin is which type of pigment?
10. Which bacteria among the following produces a water-insoluble pigment?
11. Nichrome loop wire is used in which of the following techniques?
12. Treponema sp. can be selected by taking advantage of ____________
13. Which device is used to pick a single bacterial cell from a mixed culture?
14. Which of the following method can be used to determine the number of bacteria quantitatively?
15. In pour-plate method, the medium should be maintained at what temperature?
16. The National Collection of Type Cultures is situated in ____________
17. What is the temperature of liquid nitrogen?
18. Isolation of pure culture refers to ___________
19. Vibrio cholerae can grow in a medium with a pH of _______
20. Which of the following is not a physical method for selection of pure culture?
21. Roll-tube technique is used to isolate ___________
22. Growth in broth cultures occurs mainly in the form of__________
23. In physical method of selection, endospore-forming bacteria can be obtained by heating the mixed culture to _____________
24. Pyoverdin is which type of pigment?
25. Roll-tube technique is used to isolate ___________