Cultivation of Bacteria topics include: Nutritional requirements, bacteriological media and physical conditions growth. Bacterial cultivation is a biological process that involves microorganisms multiplying themselves in a predetermined culture medium under laboratory conditions.  The exact conditions required for optimal replication will depend on the target bacterial species. The culturing parameters vary between the different bacterial species, and one of the most important factors is oxygen.  Here are some steps for culturing a bacterial species: Take a sample, such as ocean water,... Show more

Cultivation of Bacteria topics include: Nutritional requirements, bacteriological media and physical conditions growth.

Bacterial cultivation is a biological process that involves microorganisms multiplying themselves in a predetermined culture medium under laboratory conditions. 

The exact conditions required for optimal replication will depend on the target bacterial species. The culturing parameters vary between the different bacterial species, and one of the most important factors is oxygen. 

Here are some steps for culturing a bacterial species:
Take a sample, such as ocean water, soil, or spit.
Dilute the sample in water.
Spread a droplet of the dilution on a petri dish full of nutrients.
Inoculate the agar plates with the desired bacteria strain.
Store the agar plates in an incubator overnight at an optimal temperature that is suited for the bacteria's growth. 

The media must contain everything the bacteria need to survive and can be liquid or solid. Agar is added to hot liquid media to make a gel used for culturing in plates, tubes, slants, and stabs. 
Bacteria need a minimum of nutrients to grow: water, a carbon source, a nitrogen source, and some mineral salts. Water plays a fundamental role in solubilizing nutrients, transporting them, and ensuring hydrolysis reactions. Some bacteria need free water for their growth. 

Show less