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Molecular Biology Practice Test: Plasmids
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Avg score: 87% Most missed: “Which one of the following is the first engineered plasmid vector?”
Plasmids topics include: Plasmids properties, plasmid dna replicates by two alternative methods, transferring ti plasmids from bacteria to plants and plasmids helping their host cells.   A plasmid is a small, circular, double-stranded DNA molecule that is physically separated from chromosomal DNA and can replicate independently. Plasmids are most commonly found in bacteria, but can also be present in archaea and eukaryotic organisms.  Plasmids are used for a number of applications, including:  Transfection, Sequencing, Screening clones, Restriction digestion, Cloning, PCR.    There are... Show more
Molecular Biology Practice Test: Plasmids
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25 Questions

1. Which of the following types of resistance is not provided by the plasmid for its host?
2. What is the characteristic of lacZ gene of pUC18 vector among the following?
3. Transformation, transduction and conjugation occur in every bacterial cell.
4. The binary vector strategy uses two different vectors for the production of the single chimera molecule by recombination.
5. What is conjugation?
6. The host bacterium takes up a plasmid in presence of ______________
7. How many copies of special short nucleotide pair sequences for identification are present in H. influenzae and N. gonorrbacae?
8. Who were the scientists who discovered the plasmid pBR322?
9. The repressor for the β-galactosidase gene is encoded by ___________
10. The part of the bacterial plasmid incorporated in the plant genome includes ___________
11. In which year Frederic Griffith discovered transformation?
12. What is the length of the polylinker segment of plasmid?
13. In which of the plant is the crown gall disease caused?
14. Introduction of new gene through Ti plasmid should be done in ____________
15. During Frederick Griffith’s experiments what type of colonies were generated by the capsulated pneumococci?
16. What is transduction?
17. In how many states the F plasmid can occur within bacteria?
18. What is incorrect about plasmid?
19. High frequency recombination cell contains an integrated F factor. What is the name of such a cell?
20. Transformation involves the uptake of _____________
21. The main problem in the formation of the chimera Ti plasmid is _____________
22. What is the function of the capsule of the bacterial cells?
23. Transformation of E. coli does not occur.
24. How many restriction sites are contained by a plasmid?
25. Plasmid replication is dependent on the host cell.