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Molecular Biology Practice Test: Transcriptions of Genes
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Avg score: 28% Most missed: “The channel that allows the exit of the coding strand is known as ____________”
Transcriptions of Genes topics include: Genes expression by making RNA, gene recognized beginning, RNA polymerase and message manufacturing.   Gene transcription is the process of copying genes into different types of RNA. The RNA retains the information and base-pairing properties of the DNA from which it was copied.  The main enzyme involved in transcription is RNA polymerase. The stages of transcription are: Initiation: The RNA polymerase attaches to the DNA molecule and moves along it until it recognizes a promoter sequence. Elongation: The RNA polymerase adds nucleotides to the... Show more
Molecular Biology Practice Test: Transcriptions of Genes
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25 Questions

1. The E. coli RNA polymerase adds __________ nucleotides per second.
2. With respect to the formation of a complex between polymerase and DNA template which of the following is correct?
3. The RNA polymerase core enzyme converts into a holoenzyme on the addition of the _________ subunit.
4. The RNA polymerase holoenzyme has the structural formula of ___________
5. The Pribnow box is present on the coding strand of the DNA template.
6. Which of the following is not part of the RNA polymerase core enzyme?
7. The most common signal for termination of transcription is self complementation.
8. Presence of the heparin promotes transcription.
9. A stretch of which nucleotide in the DNA acts as the termination signal?
10. How many nucleotides of the growing RNA chain remain base paired with the template DNA?
11. How many proof-reading activities does RNA polymerase have?
12. –10, – 35 and +1 sites are the consensus promoter sites of sigma factor ______________
13. The eukaryotic subunit of RNA polymerase homologous to the ω subunit of bacterial RNA polymerase is ___________
14. After the binding of polymerase to the DNA template the σ factor is ejected.
15. What is the consensus sequence of the Pribnow box?
16. The β subunit of polymerase has a function of ____________
17. The Gre factor is homologous to the eukaryotic factor _____________
18. RNA polymerase adds the nucleotides de novo. The first nucleotide added by the polymerase is ____________
19. The β’ subunit of polymerase has a function of ________________
20. The rho protein can even bind within operons to terminate transcription.
21. The hairpin structure generated in the RNA is followed by a stretch of oligonucleotide complementary to the base ____________
22. The isomerisation of σ70 of bacterial polymerase is an energy dependent process.
23. The transcription bubble formed for facilitating RNA synthesis is about _____________ nucleotides long.
24. The –35 sequence is highly conserved and has a consensus sequence of ___________
25. The rho protein binds to the RNA recognizes a special structure. It consists of ____________ nucleotides.

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