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Molecular Biology Practice Test: Transcriptions of Genes
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Avg score: 28% • Most missed: “The channel that allows the exit of the coding strand is known as ____________”
Transcriptions of Genes topics include: Genes expression by making RNA, gene recognized beginning, RNA polymerase and message manufacturing.   Gene transcription is the process of copying genes into different types of RNA. The RNA retains the information and base-pairing properties of the DNA from which it was copied.  The main enzyme involved in transcription is RNA polymerase. The stages of transcription are: Initiation: The RNA polymerase attaches to the DNA molecule and moves along it until it recognizes a promoter sequence. Elongation: The RNA polymerase adds nucleotides to the... Show more
Molecular Biology Practice Test: Transcriptions of Genes
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25 Questions

1. The –10 promoter region is rich in ______________ bases.
2. Without using primers for RNA synthesis the polymerase produces small stretches of RNA of _____________ nucleotides.
3. The most common signal for termination of transcription is self complementation.
4. The Pribnow box is present on the coding strand of the DNA template.
5. The RNA polymerase can remove only the incorrect bases from the nascent RNA.
6. The isomerisation of σ70 of bacterial polymerase is an energy dependent process.
7. The α subunits of polymerase has a function of ____________
8. The complex formed by the polymerase and the promoter DNA is known as the _____________
9. The eukaryotic subunit of RNA polymerase homologous to the ω subunit of bacterial RNA polymerase is ___________
10. RNA polymerase adds the nucleotides de novo. The first nucleotide added by the polymerase is ____________
11. The rho protein can even bind within operons to terminate transcription.
12. E. coli polymerase adds upto _____________ nucleotides per second.
13. The rho protein has ____________ subunit.
14. The RNA polymerase holoenzyme has the structural formula of ___________
15. The rut sites are rich in ____________ residues.
16. How many channels are found in the RNA polymerase clamp?
17. With respect to the formation of a complex between polymerase and DNA template which of the following is correct?
18. The rho protein binds to the RNA recognizes a special structure. It consists of ____________ nucleotides.
19. Negative supercoiling enhances the rate of transcription.
20. The +1 site of conserved promoter sequence is generally a pyrimidine.
21. The –35 sequence is highly conserved and has a consensus sequence of ___________
22. After the binding of polymerase to the DNA template the σ factor is ejected.
23. The transcription bubble formed for facilitating RNA synthesis is about _____________ nucleotides long.
24. The E. coli RNA polymerase adds __________ nucleotides per second.
25. The β’ subunit of polymerase has a function of ________________

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