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Cell Biology Practice Test: Techniques in Cell Biology - Nucleic-acid Techniques
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Avg score: 40% Most missed: “Equilibrium centrifugation is also known as ___________________ centrifugation.”
Nucleic acid-based methods are highly sensitive and specific, and can be used for all categories of microbes. They can provide results quickly. Here are some nucleic acid techniques: Nucleic acid hybridization: A molecular diagnostic technique that detects the presence or absence of a specific nucleic acid sequence in a sample. It works by binding two complementary single-stranded nucleic acids together to form a double-stranded hybrid molecule. Digital PCR: A nucleic acid quantification technique that allows absolute quantification without the need for standard curves. Isothermal... Show more
Cell Biology Practice Test: Techniques in Cell Biology - Nucleic-acid Techniques
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15 Questions

1. The procedure by which DNA gel is transferred to a membrane is termed as ___________________
2. Which of the following is a commonly used label in blotting techniques?
3. One Svedberg unit is equivalent to 10-13 ______________
4. In rate-zonal sedimentation, the nucleic acids are separated base on __________________
5. In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?
6. 'Which of the following is used to lyse the nucleus and release the DNA?"
7. One Svedberg unit is equivalent to 10-13 ______________
8. Which type of gel is used for large nucleic acids?
9. Which type of gel is used for large nucleic acids?
10. Phenol is an active protein denaturant.
11. In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?
12. Equilibrium centrifugation is also known as ___________________ centrifugation.
13. Which of the following molecules can be analyzed using a northern blot?
14. Which technique can be used to separate 15N and 14N?
15. Nucleic acid hybridization is used to identify ________________________