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Molecular Biology Practice Test: Manipulation of Genetic Material
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Manipulation of Genetic Material topics include: Engineered genes and their uses, DNA and RNA libraries construction.   Genetic engineering is a process that involves manipulating an organism's DNA to alter its characteristics. This process can also be used to create new organisms.  Here are some techniques used to manipulate genetic material: Genetic engineering: Involves isolating DNA from another organism and combining it with the DNA of that individual. This process can also involve gene splicing, which introduces new genetic material into an organism's DNA. Polymerase chain reaction... Show more
Molecular Biology Practice Test: Manipulation of Genetic Material
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25 Questions

1. What is the state of phage cloning vector that is used for the construction of library?
2. Which of the following is the genetically engineered insulin?
3. The ordered steps for the construction of library involves __________i) Vector preparation
ii) Amplificationiii) Ligation and introduction into the host
iv) Isolation of genomic DNA
v) Fragmentation of DNA
4. The Insulin A vector does not contain __________
5. mRNA can be readily separated from lysed eukaryotic cells adding magnetic beads. What is the sequence of this magnetic bead?
6. The subunit vaccine for hepatitis B is created against __________
7. For the construction of DNA library what is the range of genomic DNA that has to be cloned into the vector?
8. Which enzyme is used to avoid ligation of separate DNA?
9. Active insulin consists of how many polypeptide chains?
10. The first successful active insulin was synthesized in __________
11. Which enzyme is involved in the synthesis of the DNA over an RNA template?
12. What is the significance of SI nuclease?
13. In the following type of libraries pick the odd one out.
14. What is the significance of “c” in cDNA library?
15. Pick the odd one out.
16. Monoclonal antibodies are produced by __________
17. How many types of DNA libraries are possible?
18. Synthesis of hGH is an inducer dependent process.
19. What is the characteristic of the DNA that is used for the construction of library?
20. Partial digestion is preferred for the fragmentation of DNA.
21. Pick the odd one out.
22. cDNA is synthesized from __________
23. Genetically engineered somatotropin is formed by introducing DNA fragment from the genome to the plasmid.
24. Which one of the following was first expressed as a fusion protein?
25. How many classes of interferons are found in humans?

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