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Molecular Biology Practice Test: Manipulation of Genetic Material
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Manipulation of Genetic Material topics include: Engineered genes and their uses, DNA and RNA libraries construction.   Genetic engineering is a process that involves manipulating an organism's DNA to alter its characteristics. This process can also be used to create new organisms.  Here are some techniques used to manipulate genetic material: Genetic engineering: Involves isolating DNA from another organism and combining it with the DNA of that individual. This process can also involve gene splicing, which introduces new genetic material into an organism's DNA. Polymerase chain reaction... Show more
Molecular Biology Practice Test: Manipulation of Genetic Material
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25 Questions

1. How many types of DNA libraries are possible?
2. Active insulin consists of how many polypeptide chains?
3. Which one of the following was first expressed as a fusion protein?
4. What is the significance of “c” in cDNA library?
5. Which of the following is the genetically engineered insulin?
6. Partial digestion is preferred for the fragmentation of DNA.
7. Monoclonal antibodies are produced by __________
8. The Insulin A vector does not contain __________
9. The first successful active insulin was synthesized in __________
10. What is the characteristic of the DNA that is used for the construction of library?
11. The subunit vaccine for hepatitis B is created against __________
12. Which enzyme is used to avoid ligation of separate DNA?
13. cDNA is synthesized from __________
14. Genetically engineered somatotropin is formed by introducing DNA fragment from the genome to the plasmid.
15. What is the significance of SI nuclease?
16. During storage of DNA fragments for making library, larger fragments are having more chances of getting lost.
17. For the construction of DNA library what is the range of genomic DNA that has to be cloned into the vector?
18. Which enzyme is involved in the synthesis of the DNA over an RNA template?
19. The major enzyme required for the production of a chimera molecule is __________
20. Which of the following enzymes is responsible for the digestion of the RNA strand in the RNA-DNA hybrid?
21. Pick the odd one out.
22. Synthesis of hGH is an inducer dependent process.
23. Pick the odd one out.
24. mRNA can be readily separated from lysed eukaryotic cells adding magnetic beads. What is the sequence of this magnetic bead?
25. Genetically manufactured GH is not effective for?