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Genetic Engineering Practice Test: Basics of Genetic Engineering
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Basics of Genetic Engineering topics include: Restriction endonucleases and phosphatases, polymerases, exonucleases, categories and enzymes for ligation, purification of plasmid dna, transformation, gel electrophoresis oligonucleotide and microarrays. Genetic engineering, also known as genetic modification, is a process that alters an organism's DNA. It involves using laboratory tools to insert, alter, or cut out pieces of DNA that contain one or more genes of interest.  Here are some basic concepts of genetic engineering: Recombinant DNA: A technology that involves isolating a gene from a... Show more
Genetic Engineering Practice Test: Basics of Genetic Engineering
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25 Questions

1. Which of the following is the correct nomenclature of a restriction enzyme obtained from the first activity of strain R of Escherichia coli?
2. Isoschizomers are defined as ___________
3. Ligation is defined as ______________
4. Which of the following statement is correct regarding partial digestion?
5. Which of the following activity is not possible in the case of DNA polymerase I?
6. Ligation reaction can be both intramolecular and intermolecular in nature.
7. Isocaudomers are defined as the enzymes which recognize different sequence but generate same ends. Which of the following pairs of enzymes can be termed as isocuadomers?
8. F plasmid is often used in conjugation. The correct statement is?
9. Which of the following steps is not included in the transferring of DNA?
10. At times, a specific fragment of the molecules of DNA which are analyzed needs to be separated. One of the methods is digesting the gels simply. Which of the following statement is not correct in with respect to it?
11. Phage based recombination systems are used for ____________
12. What is the kinetics of the intramolecular and intermolecular ligation reactions?
13. Electroporation is also used for taking up the DNA by the cells. It constitutes of ____________
14. What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease?
15. Blunt ends created by the restriction endonuclease can be joined.
16. Polymerase can be defined as ______________
17. Why is DNase preferred over restriction endonuclease in some cases?
18. How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends?
19. In the case DNA is transferred from gel to the membrane, it is known as ____________
20. Purification of DNA by using silica derivatized groups by DEAE is termed as ____________
21. In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?
22. Chromosomal or genomic DNA is separated by ____________
23. Oligonucleotides are synthesized chemically. Which of the following step is involved in oligonucleotide synthesis?
24. Which of the following statements is correct with respect to a unit of an enzyme?
25. Which of the following components bind to the solid column made of silica, under high salt concentration?