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Genetic Engineering Practice Test: Use of Cloned DNA
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Use of Cloned DNA topics include: Promoters, synthesis of rna and proteins, expression in vivo, fusion proteins, gene function and its traping. DNA cloning is used to create multiple copies of a gene or other piece of DNA. It can be used for a variety of purposes, including: Studying genes and their protein products: This can be important in human disease research. Creating genetically modified organisms: These can be used to study human disease, such as knock-out mice. Producing vaccines: For example, the hepatitis B vaccine. Producing transgenic plants: With improved qualities like... Show more
Genetic Engineering Practice Test: Use of Cloned DNA
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25 Questions

1. Expression of T7 promoter- lac operator hybrid requires ___________
2. If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.
3. If detection of enhancers is done it is called as ______ and for detection of transcribed sequences, it is called as ______
4. A considerable amount of modification can take place in the ______ region of RNA in _______ before translation. It affects the yield of proteins.
5. Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency.
6. Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.
7. Reticulocytes are immature red cells, which are obtained from ___________
8. What is the function of T7 lysozyme?
9. Lon protease is used for degradation of foreign proteins in E.coli cells. It is active against which type of proteins?
10. Ribosome binding site in E.coli is composed of which sequence primarily?
11. A short peptide region fused to a protein of interest is known as ___________
12. If it is desirable to express two or more proteins simultaneously, then it is known as ___________
13. Guanylyl transferase and GTP are used for?
14. RNA can be synthesized by using vector. A vector with _______ is used and further through ________ RNA is isolated.
15. Cyanogen bromide is used for cleavage of junctions. It cleaves after ________ residues.
16. Insertion of vector into intron gives rise to ________ it contains a part coming from intron and a part from ______
17. For studying processes such as splicing and cleavage, RNA is required. Choose the correct statement for this.
18. Proteins at times are not soluble in the cell and form aggregates known as ___________
19. How many methods are there, which are used for protein synthesis in vitro?
20. When lacUV5 control system is used, addition of IPTG _______ the expression of _________
21. The ______ operon encodes proteins involved in arabinose metabolism.
22. Desired changes are made in the structure of the promoter and is ligated onto reporter gene. The alteration can be known by measuring the activity.
23. The promoter can be controlled by a repressor which is temperature sensitive.
24. Maltose binding protein is the product of ______ gene in E.coli and located in ______
25. As the activity of T7 polymerase is reduced by lysosome, what is an effect on the rate of synthesis after induction?