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Genetic Engineering Practice Test: Modifications and Mutagenesis
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Modifications and Mutagenesis topics include: Insertions and deletions, mutagenesis without pcr, cre lox excision and gene disruption. Mutagenesis is a process that changes an organism's genetic information by producing a mutation. It can occur naturally, or as a result of exposure to mutagens. Mutagenesis can also be achieved in a laboratory.  Here are some differences between mutagenesis and genetic modification: Precision: Mutagenesis is less precise than genetic modification. Process: In mutagenesis, plants are exposed to radiation or toxic chemicals to scramble their genes and... Show more
Genetic Engineering Practice Test: Modifications and Mutagenesis
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25 Questions

1. Stable acquisition of the marker can take place only if a double crossover over the flanking sequence and their chromosomal counterparts causes the marker’s integration into the chromosome.
2. How can mutant strains be produced?
3. Approaches for creating mutations can be divided into how many types?
4. Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene.
5. Double stranded RNA is cleaved by a nuclease called as Dicer and small fragments are generated known as ___________
6. The process of RNA inactivation by siRNAs is termed as ___________
7. At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________
8. Cre can be introduced by crossing it with a strain containing the gene or by infection with virus containing it.
9. A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.
10. For the selection of the molecules having mutated sequence, which of the statement is true?
11. The inactivation of endogenous genes may also be helpful in directing the expression of the mutated gene in the absence of background expression of wild type gene.
12. If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________
13. The disrupted gene is excised from the vector and is inserted into the target organism. The excised gene should be in which form?
14. Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.
15. The ________ protein of bacteriophage P1 mediates site-specific recombination at a 34 bp sequence, loxP.
16. Choose the incorrect statement for the methodology of selection of molecules with mutant sequences.
17. DNA _________ is also a method for gene silencing through short RNAs.
18. An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.
19. It is often useful to inactivate endogenous genes in an organism. It might be helpful in finding out _________ role of the wild type gene.
20. Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?
21. If ______ gene is there, the double crossover may leave ________ in the chromosome.
22. RNA molecule with catalytic activity is termed as________
23. In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?
24. Excision of DNA flanked by loxP sequences is also known as _________
25. What is the function of ung gene?