The pyruvate formed during glycolysis is transported from the cytoplasm into the mitochondrial matrix where it is decarboxylated (loses a CO2) - and the acetyl group that remains is transferred to coenzyme A to form acetyl CoA; NAD+ is reduced to NAD

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1. The pyruvate formed during glycolysis is transported from the cytoplasm into the mitochondrial matrix where it is decarboxylated (loses a CO2) - and the acetyl group that remains is transferred to coenzyme A to form acetyl CoA; NAD+ is reduced to NAD