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During study, cloning of a eukaryotic gene for possible specific transcription factor is followed by insertino and ligation of gene into an expression vector. Protein translated from thsi gene is then studied on a protein blot under non denaturing conditions and probed with 32P-DNA to assess capability of encoded protein to bind to specific sequences of DNA. 1. What study technique was most likely used and why?

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The General Principles section of the USMLE covers these topics:
Biochemistry
Genetics
Microbiology
Pathology
Pharmacology


1. During study, cloning of a eukaryotic gene for possible specific transcription factor is followed by insertino and ligation of gene into an expression vector. Protein translated from thsi gene is then studied on a protein blot under non denaturing conditions and probed with 32P-DNA to assess capability of encoded protein to bind to specific sequences of DNA. 1. What study technique was most likely used and why?





 
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