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Genetic Engineering Practice Test: Use of Cloned DNA
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Use of Cloned DNA topics include: Promoters, synthesis of rna and proteins, expression in vivo, fusion proteins, gene function and its traping. DNA cloning is used to create multiple copies of a gene or other piece of DNA. It can be used for a variety of purposes, including: Studying genes and their protein products: This can be important in human disease research. Creating genetically modified organisms: These can be used to study human disease, such as knock-out mice. Producing vaccines: For example, the hepatitis B vaccine. Producing transgenic plants: With improved qualities like... Show more
Genetic Engineering Practice Test: Use of Cloned DNA
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25 Questions

1. If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.
2. In fusion proteins, the reading frame in a vector is out of phase with the insert.
3. Desired changes are made in the structure of the promoter and is ligated onto reporter gene. The alteration can be known by measuring the activity.
4. For gene trap vector system, reporter gene and _____ are present. Reporter gene is _____
5. In ________ transcription and translation is coupled.
6. Proteins at times are not soluble in the cell and form aggregates known as ___________
7. Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency.
8. Lon mutation can lead to ______ and _______
9. For co-expression of proteins, it is possible to have several independent sets of promoters and other signals in a single vector.
10. A short peptide region fused to a protein of interest is known as ___________
11. Which of the following is present in E. coli rrnB operon for rRNA?
12. Choose the correct statement for rifampcin.
13. The promoter can be controlled by a repressor which is temperature sensitive.
14. For studying processes such as splicing and cleavage, RNA is required. Choose the correct statement for this.
15. Guanylyl transferase and GTP are used for?
16. tac promoter is an example of which type of promoter?
17. Glutathione-S-Transferase (GST) enzyme is used for the conjunction of Glutathione molecules and is having a protective function in many organisms.
18. Maltose binding protein is the product of ______ gene in E.coli and located in ______
19. Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.
20. For the production of unlabelled and huge amount of proteins, which of the following is true?
21. The ______ operon encodes proteins involved in arabinose metabolism.
22. RNA can be synthesized by using vector. A vector with _______ is used and further through ________ RNA is isolated.
23. Strong secondary structure in a message may affect ________
24. Insertion of vector into intron gives rise to ________ it contains a part coming from intron and a part from ______
25. ______ fluorescent protein should be used for determination of _______ of subcellular components.

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