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Genetic Engineering Technology
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Genetic Engineering Technology
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25 Questions

1. Sequences that allows the nucleotide sequencing of cloned DNA fragments that are inserted into the plasmid

2. Retrovirus - mediated transgenics (infect mouse embryo with retrovirus and implant) - pronuclear microinjection (introduce transgenic DNA during zygote development - inject into sperm or egg) - embryonic stem cell method (embryonic stem cells mixed w

3. Chemically - Mechanically - New ways to take from levis

4. The nucleotide sequences are present in the initial mRNA transcript but are excised prior to translation

5. Isolate cells - inject gene - then reimplant cell

6. Fusion of two protoplast cells from different species: hybrid plant

7. Injection into the organism

8. Stretch of DNA with recognition sequences for various types of restriction enzymes - In plasmid - Allows for range of DNA fragments to be cloned in the plasmid

9. 1 -000-3 -000 (double- blind) - COMPARITIVE BENEFIT TO OTHER CURRENT DRUGS - confirm effectiveness - monitor side effects - compare to used treatments - and collect info -

10. Small group - 20-80 SAFETY - to establish dose range and side effects;

11. 2 -00-5 -000 base pairs

12. Pieces of DNA that can accept carry and replicate other pieces of DNA

13. Responsible for the standard of treatment for laboratory animals

14. 1-6 nucleotide repeats throughout the chromosome - 13 unique STRs used by the FBI for testing (CODIS)

15. Adult- dervied stem cells - Can differentiate but not as pluripotent as hESCs

16. Composition of matter (chemical union) - Method - Article of Manufacture - Machine

17. Each type of the enzyme cuts DNA at a specific nucleotide sequence - DNA cutting enzymes - Bind and cut at recognition sequences or restriction sites - Either create cohesive (sticky) ends or blunt ends

18. Advancement and regulation of agriculture - pests - plants and vet biologics - growing and farming practices

19. Humans are more likely to generate an unwanted immune response to the mouse version of the antibody than to the humanized version

20. Responsible for growing and farming practices

21. Expensive - have short life spans (short telomeres) - cloned from previous animals age

22. Laboratory technique uses a DNA polymerase from bacteria that grow in geysers - Allows for the exponential increase in the synthesis of specific segments of DNA

23. IPS (induced pluripotent) - ESC (embryonic stem cells - and ASC (adult stem cells

24. Short stretched of DNA with single base pair changes (wildtype) - Short length allows distinguishing which allele is present - Allows the probe to stay attached to the target DNA - Perfectly matched will cause a signal

25. Amniotic fluid- derived stem cells - Coaxed to become other cells

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