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Genetic Engineering Practice Test: Genomic Libraries
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Avg score: 77% • Most missed: “What is the final product of the RNaseH method?”
Libraries Construction topics include: Genomic libraries, cdna libraries and cloning the cDNA specialized libraries. Genomic libraries are constructed by isolating and purifying genomic DNA, generating DNA fragments, linking the fragments to a vector, and transforming the DNA into a host cell line. The main variable in constructing a genomic library is the type of vector used for the cloning of DNA fragments.  Here are the steps for constructing a genomic library: Isolate and purify genomic DNA Generate a collection of all of a species' DNA fragments Link the fragments to a... Show more
Genetic Engineering Practice Test: Genomic Libraries
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25 Questions

1. What is done after RNA fractionation is carried out?
2. In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________
3. Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?
4. Choose the correct statement with respect to the self priming method of cDNA synthesis.
5. What is the second primer in the case of 5’ RACE?
6. The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________
7. What do we mean by housekeeping genes?
8. Choose the correct statement for modification of homopolymer tailing method.
9. Choose the correct statement for RACE.
10. Libraries constructed in plasmid vectors can be kept as ___________
11. The first primer in the case of 3’ RACE is ___________
12. Choose the incorrect statement for oligo-dT cellulose.
13. Libraries can broadly be classified into how many types?
14. RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?
15. Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________
16. Choose the correct statement for genomic libraries.
17. A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________
18. Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?
19. Choose the incorrect statement for the homopolymer tailing of cDNA strands.
20. What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?
21. For cloning purposes, the intact chromosomes should be separated by ___________
22. By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.
23. Choose the incorrect statement for cDNA libraries.
24. What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides?
25. Choose the incorrect statement for the preparation of genomic libraries.

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