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Genetic Engineering Practice Test: Screening Libraries
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Avg score: 78% Most missed: “Promoter-probe vectors are used often. Choose the correct statement for these ve…”
Screening Libraries topics include: Nucleic acid hybridisation, reporter genes, coding section in vitro and screening bt expression in vivo. A screening library is a collection of compounds or fragments that are screened against a target. Screening libraries are used for drug discovery, lab drug screening, drug target identification, and other pharmaceutical-related applications.  There are different types of screening libraries, including: Peptide-encoded screening libraries: These libraries are made up of small molecules that are built up through sequential reactions. Targeted and... Show more
Genetic Engineering Practice Test: Screening Libraries
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25 Questions

1. The process of finding a particular member of the library which is having some defined properties is called as ___________
2. Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.
3. At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this?
4. If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______
5. For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.
6. If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?
7. Promoter-probe vectors are used often. Choose the correct statement for these vectors.
8. The important parameters for precise annealing of the probe and the subsequent washing don’t include ___________
9. Choose the incorrect statement for panning.
10. If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST.
11. If translation and transcription are carried out separately, then what is added to carry out translation?
12. In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody.
13. Choose the correct statement if the screening is carried out by screening by expression in vivo.
14. In ______ organisms, firstly the gene is looked into model organisms.
15. Choose the incorrect statement with respect to host used.
16. In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.
17. If screening is carried out on the basis of sequences which are related to the desired sequence, then the process is called ___________
18. Choose the incorrect statement for prehybridization mixture.
19. If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.
20. What is used for lysing of bacterial cells and denaturation of DNA?
21. If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________
22. The ligand should bind to a single protein only for the screening process.
23. Choose the incorrect statement for colony or plaque lift.
24. The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:
i) Peeling of membranes carrying away bacterial cells with it
ii) Cells are lysed and denaturing of DNA is being carried out
iii) Hybridization with labelled DNA
iv) Placing the membrane onto plate containing recombinant cells
Choose the correct sequence in which the steps are carried out (starting to ending).
25. Protein-protein interactions such as in electron transport lead to activation of the reporter gene.